DNA Extraction Experiment - 2376 Words

In this experiment for DNA extraction, series of molecular analyses conducted such as, Polymerase Chain Reaction, Polymerase Chain Reaction clean-up, Cycle Sequencing, Cycle Sequencing clean-up and some online tools and software were used to determine which animal corresponded to the unknown tissue sample obtained in the beginning of the experiment. After all the procedures, had been conducted, the unknown DNA was identified to be Cervus Elaphus. Introduction: DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. In every person’s body, DNA is identical in all the cells. Usually DNA is located in the cell’s nucleus, but a small amount of DNA can be found in the mitochondria part of the cell where it’s known as mtDNA, or mitochondrial DNA. The rate of mutation is higher in nucleic, the mtDNA is used to cut cell wall (phospholipid), ATL, and Protein K (enzyme). The information in DNA is stored as a code made up of four basses: Adenine, Guanine, Cytosine, and Thymine. The sequences of these bases determine the information available for building and maintaining an organism. The differences between individuals within and among species are mainly the result of differences in the DNA sequences that constitute the genes in their genomes. The genetic information coded in DNA is largely responsible for determining the structure, function, and development of the cell and the organism Advances in DNA (deoxyribonucleic acid) technology overShow MoreRelatedExtraction And Visualisation Of The Kiwi Fruit And Strawberry Fruit Dna1231 Words   |  5 PagesTitle: Extraction and Visualisation of the Kiwi Fruit and Strawberry Fruit DNA Introduction: All organisms have DNA (Deoxyribonucleic Acid) in the nuclei of their cells. The aim of this experiment is to extract this DNA using common household detergent to breakdown the cellular membrane then through the use of ethanol to extract the DNA from the Kiwi and Strawberry fruit. After the DNA has been extracted the DNA will be visualised by agarose gel electrophoresis which is done by adding a dye to theRead MoreBiochemical Techniques for the Extraction of Escherichia Coli Genomic DNA 845 Words   |  4 Pageswhich should be present in every biologist’s kit. This study therefore attempts to elucidate the theoretical and practical processes involved in both the polymerase chain reaction and agarose gel electrophoresis through the extraction and visualization of Escherichia coli genomic DNA through said techniques. Nucleic acids are necessary biomolecules as they function in the expression, transmission and storing of genetic information needed for the synthesis of proteins. They are responsible for passingRead MoreDNA EXTRACTION Essay1195 Words   |  5 Pageslearned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou MustRead MoreLab 13 Essay1011 Words   |  5 Pagesoriginal nucleotide sequence: RNA, DNA, Amino Acid, Protein Protein, amino acid, RNA, DNA 2. Identify two structural differences between DNA and RNA. Two structural differences between DNA and RNA would be that RNA contains ribose while DNA is missing the hydroxyl group form its ribose. Also, RNA is created by transcription of a gene locate in the cells of DNA. DNA is doubled stranded whereas RNA is single stranded. 3. Suppose you are performing an experiment in which you must use heat toRead MoreDna Extraction Lab Report1627 Words   |  7 PagesTan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube, spatula, glass rod, dropper, beaker, test tube rack, skewer. Materials : - 1 egg, meat tenderizer, salt, water , soap, isopropyl alcohol 91%, pineapple juice. Variables : Manipulated Variable : RespondingRead MoreInvestigating And Extract Chromatin From Plant And Animal Tissues1709 Words   |  7 PagesWithin this experiment, we are working to both investigate and extract chromatin from plant and animal tissues. Chromatin fundamentally is the material that makes up the chromosomes and is comprised of DNA and histone proteins. Histones, which are small proteins that encompass a large amount of the basic amino acids, enable negatively charged DNA molecules to be bound. 1 Found within eukaryotic cells’ nucleuses, histones serve to bundle DNA into nucleosomes. They make up a great deal of the componentsRead MoreHl Bio Dna Design Lab1092 Words   |  5 PagesDNA extraction in Watermelon and Bananas Abstract During this experiment the researcher investigated the differing amounts of DNA present in bananas and watermelon. So that the researcher could do this, and accurately record the amounts, the researcher liquefied the fruits in a blender, added water and salt to them, and the added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted andRead MoreGenetics Module 7 Lab 2 Essay1692 Words   |  7 Pagesï » ¿******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted:   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a. Genetic testing   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b. Body Identification   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   c. Forensic analysis 3. Name the piece ofRead MorePolymerase Chain Reaction ( Pcr )1564 Words   |  7 Pagesof the experiment, others are linked to genetic disorders and other illnesses. Batzer and Deininger (2002) even mention that â€Å"Alu insertions account for ~0.1% of all human genetic disorders (p. 374)† including some forms of cancer and diabetes. Alu, then, allows researchers to create a biological map of human activity. What are the objectives of this lab? (5 pts) Using polymerase chain reaction (PRC), this lab focuses on the amplification of DNA extracted from cheek cells. The experiment aims toRead MoreThe Extraction Of Dna From A Kiwi Fruit Run Through Agarose Gel Electrophoresis1383 Words   |  6 PagesTitle: The Extraction of DNA from a Kiwi Fruit Run Through Agarose Gel Electrophoresis. Introduction: In this experiment the use of gel electrophoresis and DNA extraction will be used to see that kiwi fruit has evidence of DNA. The aim is to be able to extract the DNA from a Kiwi Fruit then be able to load then visualize the DNA that was extracted through the use of Gel Electrophoresis. Materials: The materials used were Kiwi Fruit (peeled and cut into triangles), Plastic zip lock bag, I – 10 mL